Effective detection of remote homologues by searching in sequence dataset of a protein domain fold.
نویسندگان
چکیده
Profile matching methods are commonly used in searches in protein sequence databases to detect evolutionary relationships. We describe here a sensitive protocol, which detects remote similarities by searching in a specialized database of sequences belonging to a fold. We have assessed this protocol by exploring the relationships we detect among sequences known to belong to specific folds. We find that searches within sequences adopting a fold are more effective in detecting remote similarities and evolutionary connections than searches in a database of all sequences. We also discuss the implications of using this strategy to link sequence and structure space.
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عنوان ژورنال:
- FEBS letters
دوره 552 2-3 شماره
صفحات -
تاریخ انتشار 2003